. 2020 Apr 1;5(2):e00895-19. doi: 10.1128/mSphere.00895-19

TABLE 5.

Strains and plasmids used in this study

Strain(s) or plasmid	Characteristics^a	Source or reference
Strain(s)
E. coli DH5α	General cloning host strain	Vazyme
A. baumannii strain 19606	Wild type	ATCC
A. baumannii Ab-1, Ab-2, Ab-3, Ab-4, Ab-5, Ab-6, Ab-12, Ab-15, Ab-30, Ab-39, Ab-51	Colistin-resistant mutants derived from transposon mutant library of ATCC 19606	This study
A. baumannii Ab256-1, Ab256-2, Ab256-3, Ab256-4, Ab256-5	Colistin-resistant mutants derived from successive challenges of ATCC 19606 with increased concentrations of colistin	This study

Plasmid
pWH1266	Acinetobacter-E. coli shuttle vector, Amp^r, Tet^r	37
pSU2718	p15A, Cat^r	38
pSUTetAB	Acinetobacter-E. coli shuttle vector, Tet^r	This study
pTrc99a	pMB1, aadA, lacIq	39
pSUTetABtrc	trc promoter cloned into pSUtetAB, Tet^r	This study
pMTL-SC1	ColE1 ORI, ermB, pBP1 ORI, traJ, PtcdB, Himar1C9, catP	20
pPIC9k	Pichia pastoris expression plasmid, Amp^r, Kan^r	Invitrogen
pMarinerAb	Himar1C9- and Kan-resistant fragment cloned into pSUTetABtrc, Tet^r	This study
pAT03	pMMB67EH with FLP recombinase, Amp^r	33
pAT04	pMMB67EH with RecAb system, Tet^r	33
pMD19-T simple	General cloning vector, Amp^r	Takara
pKD4	bla FRT-kan-FRT	40
pMDK	FRT-kan-FRT cloned into pMD19-T simple	This study
pIJ773	Aac(3)IV	41
pAT03-Apr	pMMB67EH with FLP recombinase, Apra^r	This study

Amp^r, ampicillin resistance; Apra^r, apramycin resistance; Cat^r, chloramphenicol resistance; Kan^r, kanamycin resistance; Tet^r, tetracycline resistance.