FIG 10.

Effect of metal ions on the expression of MoLAEA and the genes involved in the penicillin G biosynthesis pathway. (A) The complete media were supplemented with different metal ions. The wild type was grown at 28°C and 150 rpm for 7 days. All metal ions were applied as a salt solution (5 mg/liter). (B) Quantification of penicillin G using high-performance liquid chromatography analysis of the wild-type and mutant strains 7 dpi in the absence of metal ions. (C) Quantification of penicillin G production in the presence of metal ions. (D) Fold change in expression of the homologous genes involved in the penicillin biosynthesis pathway in the absence of metal ions. (E) Fold change in expression of the homologous genes involved in the penicillin biosynthesis pathway in the presence of metal ions. Error bars represent standard deviations, and asterisks indicate statistically significant differences from the wild-type results (*, P < 0.05; **, P < 0.01). ND, not detectable; KD, knockdown strain.