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. 2020 Mar 13;23(4):100982. doi: 10.1016/j.isci.2020.100982

Figure 3.

Figure 3

CA/oPD-CPDs for Lysosomes Imaging and Intracellular pH Monitoring

(A) Cytotoxicity assay of CA/oPD-CPDs on A549 cells (n = 3, Mean ± SD).

(B–D) Confocal fluorescence images of A549 cells co-stained with CA/oPD-CPDs and LysoTracker Red excited with (B) 405 nm and (C) 536 nm and (D) overlayed images of (B) and (C) (Scale bar: 40 μm).

(E and F) Real-time intracellular pH monitoring in living A549 cells during the apoptosis process. (E) Confocal fluorescence images of A549 cells incubated with CA/oPD-CPDs during apoptosis process on a large scale (Scale bar: 40 μm). (F) Magnified confocal fluorescence images of single A549 cell during the apoptosis (Scale bar: 10 μm).

(G) PL intensity variation of CA/oPD-CPDs in living cells at different apoptosis stage (n = 6, Mean ± SD).

(H) The intracellular trafficking and transformation of CA/oPD-CPDs during apoptosis.

See also Figures S12 and S13.