Table 2.
Within-assay and between-assay coefficient of variations (CV %) of the four markers of the CELTiC panel for the three samples analysed.
| WITHIN-ASSAY |
BETWEEN-ASSAY |
WITHIN-ASSAY |
BETWEEN-ASSAY |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cq | SD | CV % | Cq | SD | CV % | ΔCq | SD | CV % | ΔCq | SD | CV % | |
| Sample 1 | ||||||||||||
| B2M | 18.2 | 0.1 | 0.3 | 17.8 | 0.3 | 1.5 | ||||||
| CEACAM6 | 33.7 | 0.4 | 1.3 | 33.5 | 0.3 | 0.9 | 15.5 | 0.4 | 2.4 | 15.6 | 0.2 | 1.3 |
| LGALS4 | 33.6 | 0.7 | 2.0 | 33.3 | 0.4 | 1.1 | 15.5 | 0.6 | 4.0 | 15.5 | 0.2 | 1.4 |
| TSPAN8 | 29.0 | 1.4 | 4.8 | 29.0 | 1.4 | 4.8 | 10.0 | 0.2 | 1.6 | 10.9 | 1.4 | 13.2 |
| COL1A2 | 29.4 | 0.4 | 1.5 | 29.2 | 1.3 | 4.6 | 10.3 | 0.3 | 3.4 | 11.0 | 1.3 | 12.2 |
| Sample 2 | ||||||||||||
| B2M | 17.1 | 0.1 | 0.8 | 17.0 | 0.2 | 1.4 | ||||||
| CEACAM6 | 33.1 | 0.1 | 0.3 | 32.9 | 0.5 | 1.4 | 15.9 | 0.1 | 0.8 | 15.9 | 0.2 | 1.5 |
| LGALS4 | 33.1 | 0.2 | 0.7 | 32.7 | 0.4 | 1.2 | 15.9 | 0.3 | 2.1 | 15.7 | 0.1 | 0.9 |
| TSPAN8 | 28.1 | 0.9 | 3.0 | 27.6 | 0.5 | 1.8 | 10.9 | 0.8 | 7.5 | 10.6 | 0.3 | 2.5 |
| COL1A2 | 28.2 | 0.1 | 0.4 | 27.8 | 0.5 | 1.8 | 11.7 | 0.8 | 6.8 | 11.1 | 0.5 | 4.6 |
| Sample 3 | ||||||||||||
| B2M | 17.1 | 0.2 | 1.0 | 17.1 | 0.1 | 0.6 | ||||||
| CEACAM6 | 30.8 | 0.3 | 1.0 | 31.1 | 0.2 | 0.6 | 13.8 | 0.2 | 1.6 | 13.9 | 0.1 | 0.8 |
| LGALS4 | 31.8 | 0.2 | 0.5 | 32.0 | 0.2 | 0.6 | 14.7 | 0.0 | 0.3 | 14.8 | 0.2 | 1.5 |
| TSPAN8 | 28.7 | 0.6 | 2.0 | 29.2 | 0.9 | 2.9 | 11.6 | 0.6 | 5.2 | 12.1 | 0.9 | 7.5 |
| COL1A2 | 28.7 | 0.3 | 1.0 | 29.6 | 1.2 | 4.0 | 11.6 | 0.4 | 3.9 | 12.4 | 1.2 | 9.9 |
| Mean values | ||||||||||||
| B2M | 17.5 | 0.1 | 0.7 | 17.3 | 0.2 | 1.2 | ||||||
| CEACAM6 | 32.5 | 0.3 | 0.8 | 32.5 | 0.3 | 1.0 | 15.1 | 0.2 | 1.6 | 15.2 | 0.2 | 1.2 |
| LGALS4 | 32.8 | 0.3 | 1.0 | 32.7 | 0.3 | 0.9 | 15.4 | 0.3 | 2.1 | 15.4 | 0.2 | 1.3 |
| TSPAN8 | 28.6 | 1.0 | 3.4 | 28.6 | 0.9 | 3.2 | 10.9 | 0.5 | 4.8 | 11.2 | 0.9 | 7.7 |
| COL1A2 | 28.8 | 0.3 | 1.0 | 28.9 | 1.0 | 3.5 | 11.2 | 0.5 | 4.7 | 11.5 | 1.0 | 8.9 |
B2M, beta 2 microglobulin; CEACAM6, carcinoembryonic antigen-related cell-adhesion molecule 6; LGALS4, lectin galactoside-binding soluble 4; TSPAN8, tetraspanin 8; COL1A2, collagen type I alpha 2 chain; ΔCq, mean quantification cycles after normalisation on housekeeping gene B2M; SD, standard deviation.