Table 3.

Influenza A viruses identified by LC–MS/MS.

Strains	Subtype	Titera	Sequence coverageb (%)					Identified asc
			NP	M1	NS1	HA	NA
BM1454-55	H3N2	109	70	75	56	21	15	Influenza A H3N2
		108	49	47	34	17	11
		107	25	18	0	2	0
		106	10	0	0	0	0
BM1456-57	H1N1	109	72	71	61	18	5	Influenza A H1N1
BM1480-85		108	56	49	39	10	4
		107	44	30	15	0	0
		106	14	0	0	0	0

^aTenfold dilution series of each tested influenza strain was prepared and analyzed by LC–MS/MS. Indicated are the total genome copies of the analyzed influenza virus subjected to LC–MS/MS.

^bAmino acid sequence coverage (%) of proteins determined by MASCOT, as based on identified peptides. Percentages are the averages of combined results for the H3N2 or H1N1 strain. Proteins were considered significantly identified when the MASCOT score was ≥50 and when a minimum of three peptides each with a score of ≥20 was identified. NP, nucleoprotein; M1, matrix protein; NS1, non-structural protein 1; HA, hemagglutinin; NA, neuraminidase;

^cIdentification of type and subtype based on the highest MASCOT scores of nucleoprotein; thus, the given subtypes should be considered as indicative.