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. 2014 Dec 8;213:75–83. doi: 10.1016/j.jviromet.2014.11.014

Table 5.

Identified proteins of two different respiratory viruses from mixed samples. Mixture A contained H3N2 (BM1454) and RSV (BM1450). Mixture B contained H1N1 (BM1456) and hMPV (BM1460).

Mix Titera Id. virusb Average sequence coverage of identified proteinsc (%)
NP M1 NS1 HA NA M2-1 F P
A 109 Influenza A/H3N2 63 74 16 15 4
RSV 21 43 53 9 55
108 Influenza A/H3N2 48 49 20 13 7
RSV 23 40 46 3 53
107 Influenza A/H3N2 18 34 0 5 0
RSV 14 0 23 0 0



B 109 Influenza A/H1N1 73 67 60 0 0
hMPVd 14 29 10 15 16
108 Influenza A/H1N1 49 33 39 0 0
hMPVd 8 18 10 9 18
107 Influenza A/H1N1 24 10 11 0 0
hMPVd 0 0 0 0 0
a

Total genome copy number of the tested influenza virus and RSV in a total sample volume subjected to LC–MS/MS analysis.

b

Identified virus.

c

An average sequence coverage (%) of the identified protein was calculated from two independent experiments. A protein was considered identified when the MASCOT score was ≥50 and when a minimum of three peptides with a score ≥20 was identified in the protein.

d

The starting concentration of hMPV was 100 times lower than that for influenza and RSV.

NP, nucleoprotein; M1, matrix protein; NS1, non-structural protein 1; HA, hemagglutinin; NA, neuraminidase; M2-1, matrix protein; F, fusion glycoprotein; P, phosphoprotein.