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. 2014 Dec 8;213:75–83. doi: 10.1016/j.jviromet.2014.11.014

Table 6.

Identification of viral proteins from reconstituted clinical material spiked with viruses.

Virus Titera Average sequence coverage of identified proteinsb (%)
NP M1 NS1 HA NA PA M2-1 F P
H3N2 109c 69 71 32 13 12 0 ND ND ND
108 25 27 0 0 0 0 ND ND ND
107 4 4 0 0 0 0 ND ND ND



H1N1 109c 68 77 71 0 7 3 ND ND ND
108 24 14 9 0 0 0 ND ND ND



RSV 109c 54 54 27.3 ND ND ND 35 9 31
108 3 35 ND ND ND ND 15 0 17



hMPV 107c 25 48 ND ND ND ND 16 28 19
106 0 0 ND ND ND ND 0 0 6d



Neg. control Throat swab 0 0 0 0 0 0 0 0 0
a

Total genome copy number of the tested influenza virus and RSV in a total sample volume subjected to LC–MS/MS analysis.

b

An average sequence coverage (%) of the identified protein was calculated from two independent experiments. A protein was considered identified when the MASCOT score was ≥50 and when a minimum of two peptides with a score ≥20 was identified in the protein. NP, nucleoprotein; M1, matrix protein; NS1, non-structural protein 1; HA, hemagglutinin; NA, neuraminidase; M2-1, matrix protein; F, fusion glycoprotein; P, phosphoprotein.

c

Positive control; isolation of the virus was without throat swab material.

d

Only in one of the two tested samples peptides were detected.