Fig. 1.

Consensus sequence alignment of virulent PEDVs of Colorado (accession no. KF272920), IA1 (accession no. KF468754), IA2 (accession no. KF468A753), MN (accession no. KF468752), OH1414 (accession no. KJ408801) isolates and variant (v) PEDV OH851 (accession no. KJ399978) isolates in the amplified region, including the primer and probe target sequences (bold). Red triangles indicate the three deletion sites, and red square indicates the insertion site. Names of primers and probes are indicated in the corresponding regions. Nucleotide numbering of virulent PEDV and variant PEDV is indicated based on their complete genome sequences.