Table 2.
Specificity of the duplex real-time RT-PCR assay#.
| Virus name | Result | Note | |
|---|---|---|---|
| Intra-specificity | Virulent PEDV OH1715 (variant PEDV probe) | Negative | Single probe used |
| Variant PEDV OH851 (Virulent PEDV probe) | Negative | ||
| Inter-specificity | Transmissible gastroenteritis virus* | Negative | Two probes used |
| Porcine coronavirus HKU15 KY4813 | Negative | ||
| Porcine reproductive and respiratory syndrome virus OH28372 | Negative | ||
| Swine influenza virus OH27361(H3N2) | Negative | ||
| Encephalomyocarditis virus* | Negative | ||
| Porcine parvovirus* | Negative | ||
| Pseudorabies virus* | Negative |
#
Positive controls were run in the duplex real-time using RNA samples of both OH1715 and OH851; Negative control were run using distilled water.
*
Virus strains purchased from National Veterinary Service Laboratory.