Table 1.
Composition of stabilizing formulations used during freeze-drying of HCoV NL63 samples. Composition of different formulations including lyoprotectants, bulking agents, additional excipients and references to original works are included in the table. All formulas are presented for 1× concentrated solutions. Formulation names are used throughout the manuscript.
| Lp. | Name | Lyoprotectant | Bulking agent | Reference |
|---|---|---|---|---|
| 1. | In25 | Inulin 25 g/l | – | Audouy et al. (2011), de Jonge et al. (2007), Geeraedts et al. (2010), Amorij et al. (2008) |
| 2. | In40 | Inulin 40 g/l | – | |
| 3. | Treh100 | Trehalose 100 g/l | – | Croyle et al. (1998), Bieganski et al. (1998), Amorij et al. (2008) |
| 4. | Sucr0.5 | Sucrose 0.5 M (171 g/l) | – | Croyle et al. (1998) |
| 5. | Sucr74.6 | Sucrose 74.6 g/l | Gelatin 2% | Levy and Fieldsteel (1982) |
| 6. | Sor10 | Sorbitol 10% | Gelatin 0.4% | Croyle et al. (1998) |
| 7. | Sor2 | Sorbitol 2% | Gelatin 0.5% | Kang et al. (2010) |
| 8. | 50Mia | Myo-inositol 50 g/l | – | Burger et al. (2008) |
| 9. | 35Mi15Manna | Myo-inositol 35 g/l | 15 g/l Mannitol | |
| 10. | 25Mi25Manna | Myo-inositol 25 g/l | 25 g/l Mannitol | |
| 11. | 35Mi15Sora | Myo-inositol 35 g/l | 15 g/l Sorbitol | |
| 12. | 50Mi2Leua | Myo-inositol 50 g/l | 2 g/l Leucine | |
| 13. | 30Mi15Gela | Myo-inositol 30 g/l | 15 g/L Gelatin |
a
Samples supplemented with gelatin (25 g/l; with the exception of formulation 13), L–arginine (16 g/l), alanine (1 g/l), and histidine (2.1 g/l).