Table 1.

Primers and probes of various PEDV and PDCoV RT-PCRs evaluated in this study.

Assay name	Primer & Probe	Nucleotide sequence (5′–3′)a	Nucleotide Position	Target gene	Amplicon	Reference
PEDV RT-iiPCR	PEDV iiF	AATAGCATTCGGTTGTGGCG	25978–25997	M	89 bp	This paper
	PEDV iiR	CGGCCCATCACAGAAGTAGT	26066–26047
	PEDV iiP	FAM-CATTCTTGG/ZEN/TGGTCTTTCAATCCTGA-IABkFQ	26005–26030
PDCoV RT-iiPCR	PDCoV iiF	GAGAGTAGACTCCTTGCAGGGATTAT	23356–23381	M	106 bp	This paper
	PDCoV iiR	GCTTGCCATGCTTAACGACTG	23461–23441
	PDCoV iiP	FAM-AATGCACCTCCATGTACC-MGB	23409–23392
PEDV/PDCoV duplex real-time RT-PCRa	PEDV rF	GGTTGTGGCGCAGGACA	25988–26004	M	79 bp	This paper
	PEDV rR	CGGCCCATCACAGAAGTAGT	26066–26047
	PEDV rP	FAM-CATTCTTGG/ZEN/TGGTCTTTCAATCCTGA-IABkFQ	26005–26030
	PDCoV rF	TGAGAGTAGACTCCTTGCAGGGA	23355–23377	M	105 bp
	PDCoV rR	GAGAATTGGAGCCATGTGGT	23436–23417
	PDCoV rP	NED-TGTACCCATTGGATCCATAA-MGB	23397–23378
PEDV real-time RT-PCR	PEDV-F	CGCAAAGACTGAACCCACTAACCT	26684–26707	N	198 bp	Lowe et al. (2014)
	PEDV-R	TTGCCTCTGTTGTTACTTGGAGAT	26881–26858
	PEDV-P	FAM-TGTTGCCAT/ZEN/TACCACGACTCCTGC-IABkFQ	26847–26824
PDCoV real-time RT-PCR	PDCoV-F	CGACCACATGGCTCCAATTC	23415–23434	M	70 bp	Chen et al. (2015)
	PDCoV-R	CAGCTCTTGCCCATGTAGCTT	23484–23464
	PDCoV-P	FAM-CACACCAGT/ZEN/CGTTAAGCATGGCAAGC-IABkFQ	23436–23461

^aNucleotide positions of PEDV and PDCoV primers and probes are based on GenBank accession no. KF650371 and KJ567050, respectively.