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. 2016 Apr 6;234:34–42. doi: 10.1016/j.jviromet.2016.03.016

Table 3.

Analytical sensitivity of PEDV RT-iiPCR and duplex PEDV/PDCoV real-time RT-PCR for detection of PEDV RNA and comparison with the reference singleplex PEDV real-time RT-PCR using viral RNA from the serially diluted PEDV cell culture isolates. (For interpretation of the references to color in this table legend, the reader is referred to the web version of this article.)

graphic file with name fx2.gif

Note: Nucleic acids were extracted from all samples using the MagMAX™ Pathogen RNA/DNA Kit and Kingfisher-96 instrument from Thermo Fisher Scientific.