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. 2016 Apr 6;234:34–42. doi: 10.1016/j.jviromet.2016.03.016

Table 4.

Analytical sensitivity of PDCoV RT-iiPCR and duplex PEDV/PDCoV real-time RT-PCR for detection of PDCoV RNA and comparison with the reference singleplex PDCoV real-time RT-PCR using viral RNA from the serially diluted PDCoV cell culture isolate. (For interpretation of the references to color in this table legend, the reader is referred to the web version of this article.)

graphic file with name fx3.gif

Note: Nucleic acids were extracted from all samples using the MagMAX™ Pathogen RNA/DNA Kit and Kingfisher-96 instrument from Thermo Fisher Scientific.