Table 5.
Specimen types of 170 clinical samples and Ct ranges of positive samples tested by the reference PEDV rRT-PCR and PDCoV rRT-PCR.
| Specimen type | Number | Positive by the reference PEDV rRT-PCR |
Positive by the reference PDCoV rRT-PCR |
Positive by both the reference PEDV and PDCoV rRT-PCRs | ||
|---|---|---|---|---|---|---|
| Number | Ct ranges | Number | Ct ranges | Number | ||
| Fecal swab | 86 | 47 | 14.1–30.6 | 37 | 16.7–35.6 | 5 |
| Feces | 24 | 9 | 12.2–30.8 | 17 | 15.0–28.3 | 2 |
| Intestine | 19 | 10 | 16.2–34.1 | 9 | 17.2–31.7 | 0 |
| Oral fluid | 41 | 22 | 14.6–35.7 | 19 | 20.3–37.1 | 9 |
Note: Nucleic acids were extracted from all samples using the MagMAX™ Pathogen RNA/DNA Kit and Kingfisher-96 instrument from Thermo Fisher Scientific.