Table 6.

Sensitivity and specificity of PEDV RT-iiPCR, PDCoV RT-iiPCR, and duplex PEDV/PDCoV rRT-PCR as compared to the reference singleplex PEDV rRT-PCR and PDCoV rRT-PCR based on testing 170 clinical samples.

		Reference PEDV rRT-PCR or reference PDCoV rRT-PCR
		Positive	Negative	Total
(a) PEDV RT-iiPCR compared to the reference singleplex PEDV rRT-PCR
PEDV RT-iiPCR	Positive	86	1	87
	Negative	2	81	83
	Total	88	82	170
Sensitivity: 97.73%; Specificity: 98.78%; Accuracy: 98.24%
(b) Duplex PEDV/PDCoV rRT-PCR compared to the reference singleplex PEDV rRT-PCR
Duplex PEDV/PDCoV rRT-PCR	Positive	87	3	90
	Negative	1	79	80
	Total	88	82	170
Sensitivity: 98.86%; Specificity: 96.34%; Accuracy: 97.65%
(c) PDCoV RT-iiPCR compared to the reference singleplex PDCoV rRT-PCR
PDCoV RT-iiPCR	Positive	82	0	82
	Negative	0	88	88
	Total	82	88	170
Sensitivity: 100%; Specificity: 100%; Accuracy: 100%
(d) Duplex PEDV/PDCoV rRT-PCR compared to the reference singleplex PDCoV rRT-PCR
Duplex PEDV/PDCoV rRT-PCR	Positive	79	0	79
	Negative	3	88	91
	Total	82	88	170
Sensitivity: 96.34%; Specificity: 100%; Accuracy: 98.24%

Notes: (1) Nucleic acids were extracted from all samples using the MagMAXTM Pathogen RNA/DNA Kit and Kingfisher-96 instrument from Thermo Fisher Scientific. (2) The reference PEDV rRT-PCR and PDCoV rRT-PCR: Ct < 40 positive. (3) The duplex PEDV/PDCoV rRT-PCR: Ct < 40 positive; Ct ≥ 40 negative.