Table 8.
Sensitivity and specificity of PEDV RT-iiPCR and PDCoV RT-iiPCR as compared to the reference singleplex PEDV rRT-PCR and PDCoV rRT-PCR based on testing 170 clinical samples with different nucleic acid extraction methods.
| (a) Comparison of PEDV RT-iiPCR and the reference singleplex PEDV rRT-PCR | ||||
|---|---|---|---|---|
| MagMAX™ extraction and reference PEDV rRT-PCR |
||||
| Positive | Negative | Total | ||
| taco™ mini extraction and PEDV RT-iiPCR | Positive | 83 | 0 | 83 |
| Negative | 5 | 82 | 87 | |
| Total | 88 | 82 | 170 | |
| Sensitivity: 94.32%; Specificity: 100%; Accuracy: 97.06% | ||||
| (b) Comparison of PDCoV RT-iiPCR and the reference singleplex PDCoV rRT-PCR | ||||
|---|---|---|---|---|
| MagMAX™ extraction and reference PDCoV rRT-PCR |
||||
| Positive | Negative | Total | ||
| taco™ mini extraction and PDCoV RT-iiPCR | Positive | 82 | 0 | 82 |
| Negative | 0 | 88 | 88 | |
| Total | 82 | 88 | 170 | |
| Sensitivity: 100%; Specificity: 100%; Accuracy: 100% | ||||