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. 2014 Dec 24;115:9–16. doi: 10.1016/j.antiviral.2014.12.011

Table 1.

Structure and IC50 of compounds against coronaviral PLpro.

Name Structure IC50 (μM)a
Peptide cleavage DUB activity
MERS-CoV PLpro
6-Mercaptopurine (6MP) graphic file with name fx1.gif 26.9 ± 7.5 25.8 ± 5.0
6-Thioguanine (6TG) graphic file with name fx2.gif 24.4 ± 4.3 12.4 ± 1.9
N-ethylmaleimide (NEM) graphic file with name fx3.gif 45.0 ± 10.1 NDb
Mycophenolic acid graphic file with name fx4.gif MERS-CoV PLpro
247.6 ± 55.8 222.5 ± 57.8
SARS-CoV PLpro
No inhibition No inhibition
SARS-CoV PLpro E168R
260.1 ± 21.0 NDb
a

The peptide substrate (Dabcyl–FRLKGGAPIKGV–Edans) at 20 μM or Ub-AFC at 0.5 μM were used for the IC50 assay, respectively. All the assays were repeated several times to ensure reproducibility.

b

Not determined.