Table 2.

Kinetic parameters of 6MP, 6TG, and mycophenolic acid inhibition of MERS-CoV PL^pro.

PLpro in	Km (μM)	kcat (min−1)	kcat/Km (10−3min−1 μM−1)	Kis (μM)
pH 6.5a	19.2 ± 2.6	0.24 ± 0.01	12.5 ± 1.9
6MPb	21.9 ± 3.3	0.28 ± 0.02	12.8 ± 2.0	14.3 ± 1.6
6TGb	14.1 ± 2.3	0.23 ± 0.02	16.3 ± 3.0	9.1 ± 1.2
Mycophenolic acidc	15.2 ± 1.1	0.26 ± 0.01	17.1 ± 1.4	263.7 ± 13.8

^aThe values were from our previous studies (Lin et al., 2014).

^bIn the presence of 6MP or 6TG, the steady-state kinetic data were determined by the competitive inhibition model (Eq. (1)) (Fig. 2A and B).

^cIn the presence of mycophenolic acid, the kinetic data were determined by the noncompetitive inhibition model (Eq. (2)) (Fig. 2C).