Table 2.

Analytical sensitivity comparison of the multiplex assay and singular assays.

		PCV3		PCV2
		Singular	Multiplex	Singular	Multiplex
Efficiency (E)		94.7%	92.9%	96.9%	95.3%
Correlation coefficient (R2)		0.994	0.995	0.998	0.998
Mean Cts of 3 replicates of clinical samples at different dilutions	100	17.14	17.13	17.14	17.07
	10−1	20.69	20.70	21.24	20.62
	10−2	24.37	23.95	24.16	23.86
	10−3	28.54	27.23	27.48	27.17
	10−4	31.73	30.74	30.81	30.64
	10−5	34.92	34.89	34.30	34.79
	10−6	37.10	37.44	37.94	37.36