Table 1.
Comparison of individual PCR assays included in Respanel against different reference methods.
| PCR assay | Sample description | Reference methods | Total number of samples | TP | FP | TN | FN | Sensitivity (%) | Specificity (%) | PPV (%) | NPV (%) | Accuracy (%) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Adenovirus | QCMD EQA specimens ATCC Clinical samples |
Culture PCR Resplex |
44 | 20 | 1 | 20 | 3 | 87 | 95 | 95 | 87 | 91 |
| Human Metapneumovirus | QCMD EQA specimens CAP EQA specimens Clinical samples |
PCR DFA |
24 | 10 | 0 | 14 | 0 | 100 | 100 | 100 | 100 | 100 |
| Influenza A | QCMD EQA specimens Clinical samples |
PCR DFA Resplex |
28 | 17 | 0 | 10 | 1 | 94 | 100 | 100 | 91 | 96 |
| Influenza B | QCMD EQA specimens Clinical samples |
DFA Resplex |
42 | 11 | 0 | 31 | 0 | 100 | 100 | 100 | 100 | 100 |
| Parainfluenza virus 1 | CAP EQA specimens Clinical samples |
Culture DFA Resplex |
38 | 10 | 0 | 27 | 1 | 91 | 100 | 100 | 96 | 97 |
| Parainfluenza virus 2 | CAP EQA specimens Clinical samples |
Culture DFA Resplex |
38 | 7 | 0 | 30 | 1 | 88 | 100 | 100 | 97 | 97 |
| Parainfluenza virus 3 | CAP EQA specimens Clinical samples |
Culture DFA Resplex |
33 | 12 | 0 | 19 | 2 | 86 | 100 | 100 | 90 | 94 |
| Respiratory Syncytial Virus | QCMD EQA specimens Clinical samples |
DFA Resplex |
52 | 29 | 1 | 22 | 0 | 100 | 96 | 97 | 100 | 98 |
| Enterovirus | QCMD EQA specimens ATCC Clinical samples |
Culture PCR Resplex |
44 | 24 | 0 | 17 | 3 | 89 | 100 | 100 | 85 | 93 |
| Rhinovirus | QCMD EQA specimens ATCC Clinical samples |
Culture Resplex |
44 | 12 | 0 | 32 | 0 | 100 | 100 | 100 | 100 | 100 |
| Bocavirus | Clinical samples | Resplex | 30 | 14 | 0 | 16 | 0 | 100 | 100 | 100 | 100 | 100 |
| Coronavirus 229E | Clinical samples | Resplex | 22 | 3 | 0 | 17 | 2 | 60 | 100 | 100 | 89 | 91 |
| Coronavirus OC43 | Clinical samples | Resplex | 22 | 1 | 0 | 21 | 0 | 100 | 100 | 100 | 100 | 100 |
| Coronavirus NL63 | Clinical samples | Resplex | 22 | 7 | 0 | 15 | 0 | 100 | 100 | 100 | 100 | 100 |
| Coronavirus HKU1 | Clinical samples | Resplex | 22 | 4 | 0 | 18 | 0 | 100 | 100 | 100 | 100 | 100 |
| Mycoplasma pneumoniae | ATCC Clinical samples |
Culture Resplex |
52 | 14 | 0 | 38 | 0 | 100 | 100 | 100 | 100 | 100 |
| Streptococcus pneumoniae | ATCC Clinical samples |
Culture PCR |
36 | 9 | 0 | 27 | 0 | 100 | 100 | 100 | 100 | 100 |
| Chlamydophila pneumoniae | ATCC Clinical samples |
Culture | 52 | 3 | 0 | 49 | 0 | 100 | 100 | 100 | 100 | 100 |
| Bordetella pertussis | QCMD EQA specimens ATCC Clinical samples |
Culture PCR |
125 | 25 | 1 | 98 | 1 | 96 | 99 | 96 | 99 | 98 |
QCMD = Quality Control for Molecular Diagnostics, CAP = College of American Pathologists, EQA = External Quality Assessment, ATCC = American Type Culture Collection, DFA = Direct Fluorescent Antibody, TP = True positive, FP = False positive, TN = True negative, FN = False negative, PPV = Positive predictive value, NPV = Negative predictive value. Sensitivity, specificity, PPV, NPV and accuracy were calculated as defined in the materials and methods (Section 2.6).