Table 1.
The primers of the plasmids used in the study (the restriction endonucleases sites were underlined and italic, T7 promoter and tobramycin aptamer tag sequences were underlined in the forward and reverse primers).
| Plasmids | Primers |
|---|---|
| pCold-I-DDX5/pGEX-4T-3-DDX5 | Forward primer: GATACTAGGATCCATGTCGGGTTATTCGAG |
| Reverse primer: GAGCTCGGTCGACTTATTGGGAATATCCTG | |
| pcDNA3.1-DDX5 (K144E and S279L)-HA |
Forward primer: GTCATGCAAGCTTGCCACCATGTCGGGTTA TTCGAGTGAC |
| Reverse primer: GTAGTTAGGATCCCTAGGCGTAGTCGGGGA CGTCGTAGGGGTATTGGGAATATCCTGTT | |
| pcDNA3.1-core-MYC | Forward primer: GCCGCCAAGCTTGCCACCATGATGACTAAA AAACCAGGAGGGCC |
| Reverse primer: GCGGGCGGGATCCCTACAAATCTTCTTCAG AAATCAACTTCTGTTCGGCTCCTGCACAA | |
| pcDNA3.1-NS3-MYC | Forward primer: GTATTATGGTACCGCCACCATGGGGGGCGT GTTTTGGGAC |
| Reverse primer: GCGGGCGCTCGAGCTACAAATCTTCTTCAG AAATCAACTT CTGTTCTCTCTTCCCTGCT | |
| pcDNA3.1-NS5 (Mtase) -MYC |
Forward primer: GATATATGGTACCGCCACCATGGGAAGGCC TGGGGG |
| Reverse primer: GCGGGCGCTCGAGCTACAAATCTTCTTCAG AAATCAACTTCTGTTCTCCCACGGCTCTT | |
| pcDNA3.1-NS5 (RdRp) -MYC |
Forward primer: GACATATGGTACCGCCACCATGTCAGCC AGCTCTCTC |
| reverse primer: GCGGGCGCTCGAGCTACAAATCTTCTTCAG AAATCAACTTCTGTTCGATGACCCTGTCT | |
| DDX5-K144E (Quikchange) | Forward primer: GTGGCACAGACTGGATCTGGGGAGACATT GTCTTATTTGCTTCCT |
| Reverse primer: AGGAAGCAAATAAGACAATGTCTCCCCA GATCCAGTCTGTGCCAC | |
| DDX5-S279L (Quikchange) |
Forward primer: GATAGGCAAACTCTAATGTGGCTAGCGAC TTGGCCAAAAG AAGTA |
| Reverse primer: TACTTCTTTTGGCCAAGTCGCTAGCCACA TTAGAGTTTGCCTATC | |
| pET-24a-core | Forward primer: GCCGCCGAATTCATGACTAAAAAACCAG |
| Reverse primer: ATAATACTCGAGGGCTCCTGCACAAGCTAT | |
| pET-24a-NS3 | Forward primer: GACGACACATATGGGGGGCGTGTTTTGGGA |
| Reverse primer: GCGCGCGCTCGAGTCTCTTCCCTGCTGCAA | |
| pET-24a-NS5-MTase | Forward primer: GATTTACATATGGGAAGGCCTGGGGGCAG |
| Reverse primer: GCTCATACTCGAGTCCCACGGCTCTTGTTCC | |
| pET-24a-NS5-RdRP | Forward primer: GATATGCGGATCCTCAGCCAGCTCTCTCGT |
| Reverse primer: GGCCGACTCGAGGATGACCCTGTCTTCCTGG ATCAAGACG | |
| pcDNA3.1-DDX5r, pcDNA3.1-DDX5r- (K144E,S279L)-HA (Quikchange) | Forward primer: TAAGACCTGATAGGCAAACCTTGATGTGGTC CGCTACTTGGCCAAAAGAAGTAAGA |
| Reverse primer: TCTTACTTCTTTTGGCCAAGTAGCGGACCAC ATCAAGGTTTGCCTATCAGGTCTTA | |
| JEV 5′ UTR | Forward primer: TAATACGACTCACTATAGGGAGAAGTTTATC TGTGTGAACTTCTTGGCTTA |
| Reverse primer: GGCTCAGCACGAGTGTAGCTAAACCTCGCTA TACTAAGCCGGTTATCTTCCGTTCTAA | |
| JEV 3′ UTR | Forward primer: TAATACGACTCATAGGGTAGTGTGATTTAAG GTAGAAAAGTAG |
| Reverse primer: GGCTCAGCACGAGTGTAGCTAAACCTCGCTA TACTAAGCCAGATCTTGTGTTCTTCCT | |
| Ampicillin resistance gene (1–250 bp) |
Forward primer: TAATACGACTCACTATAGGGATGAGTATTCA ACATTTCCGTGTCG |
| Reverse primer: GGCTCAGCACGAGTGTAGCTAAACCTCGCTA TACTAAGCCCGTCAATACGGGATAATAC | |
| pGEX-4T-3-core | Forward primer: GCCGCCGGATCCATGACTAAAAAACCAG |
| Reverse primer: ATAATACTCGAGGGCTCCTGC ACAAGCTAT | |
| pGEX-4T-3-NS3 | Forward primer: GACGACAGAATTCGGGGGCGTGTTTTGGGA |
| Reverse primer: GCGCGCGCTCGAGTCTCTTCCCTGCTGCAA | |
| pGEX-4T-3-NS5-MTase | Forward primer: GATTTAGAATTCGGAAGGCCTGGGGGCAG |
| Reverse primer: GCTCATACTCGAGTCCCACGGCTCTTGTTCC | |
| pGEX-4T-3-NS5-RdRP | Forward primer: GATATGCGGATCCTCAGCCAGCTCTCT CGT |
| Reverse primer: GGCCGACTCGAGGATGACCCTGTCTTCCTGG ATCAAGACG |