Table 1.
Sequence of the oligonucleotides used for PCR.
| Primer Name | Sequence (5′-3′)a |
|---|---|
| HR1-F | GTCGGATCCCTCAATTATCTTGCTC |
| HR1-R | CCGCTCGAGTCACTCATTAACCTTTTGCTGTGC |
| HR1M-F | GTTGGATCCCGGAACCAGCAATTG |
| HR1M-R | ATACTCGAGTCATTGCAGCTGTACGGT |
| HR1L-F | GTTGGATCCTCTGCTATTGGTAAT |
| HR1L-R | ATACTCGAGTCATTGGAAGTTGTGTTG |
| HR2-F | GTTGGATCCAAGCTCACTGGTGA |
| HR2-R | ATACTCGAGTCAGAGCCACTCAAGGT |
| HR2M-F | GTCGGATCCAATGCCACTTATCTTA |
| HR2M-R | ATACTCGAGTCAAAGGTCAACTAGTGTG |
| HR2L-F | GTCGGATCCCCTTTAGATGTTTTTA |
| HR2L-R | ATACTCGAGTCATGTCTCAACTCGGT |
| HR2P-F | GTCGGATCCAATGCCACTTATCTTA |
| HR2P-R | ATACTCGAGTCACTCAACTCGGTTGAG |
a
Restriction endonuclease sites: BamHI (italic) and XhoI (underlined).