Figure 2.

PLK1 subcellular distribution and function during metaphase in mammalian cells. (a) PLK1 (orange) localizes during M‐phase to the mitotic centrosomes, kinetochores, and cytokinetic midbody (magenta) to ensure mitotic progression, microtubule attachments, and anaphase onset, as well as proper cytokinesis and abscission. Gradient below (orange) represents relative PLK1 activity changes between prometaphase/metaphase and cytokinesis. (b) Table outlining PLK1 localization patterns with corresponding functions and known binding scaffolds during M‐phase