Table 1.
Primers used in real‐time polymerase chain reaction gene expression analysis
| Gene | Primer sequences (5′‐3′) |
|---|---|
| GAPDH | |
| Forward | GCATCTCCCTCACAATTTCCA |
| Reverse | TGCAGCGAACTTTATTGATGGT |
| ALP | |
| Forward | AACCCAGACACAAGCATTCC |
| Reverse | GCCTTTGAGGTTTTTGGTCA |
| RUNX2 | |
| Forward | TTCTCCAACCCACGAATGCAC |
| Reverse | CAGGTACGTGTGGTAGTGAGT |
| OCN | |
| Forward | CGCTCTGTCTCTCTGACCTC |
| Reverse | TCACAAGCAGGGTTAAGCTC |
| COL‐1 | |
| Forward | AATGGTGCTCCTGGTATTGC |
| Reverse | GGCACCAGTGTCTCCTTTGT |
| IL‐17RA | |
| Forward | TCAGGGCCAGTGTGAAAACA |
| Reverse | CATGTCGGGTTATCAGGGAAA |
| IL‐6 | |
| Forward | TAGTCCTTCCTACCCCAATTTCC |
| Reverse | TTGGTCCTTAGCCACTCCTTC |
| IL‐1β | |
| Forward | GCAACTGTTCCTGAACTCAACT |
| Reverse | ATCTTTTGGGGTCCGTCAACT |
Abbreviations: ALP, Alkaline phosphatase; COL, collagen; IL, interleukin; OCN, osteocalcin; RA, receptor A; RUNX2, runt‐related transcription factor 2.