Table 1.
CC50, EC50 and EC90 values determined for silvestrol-treated cells that were mock infected (CC50) or infected with the indicated viruses (EC50/EC90). MTT assays were performed at 37°C except for cells used for HCoV-229E and HRV A1 infections, which were performed at 33°C (indicated by asterisks). SI, selectivity index. Experiments were done in biological triplicate.
| Cells | Virus | CC50 [μM] | EC50/EC90 [nM] | SI |
|---|---|---|---|---|
| MRC-5 | HCoV-229E | >10* | 3/27 | >3330 |
| MRC-5 | MERS-CoV | >10 | 1.3/12 | >7690 |
| MRC-5 | HRV A1 | >10* | 100/900 | >100 |
| MRC-5 | PV | >10 | 20/180 | >500 |
| PBMCs | HCoV-229E | >1* | 2.8/25 | >350 |
| Huh-7 | HCoV-229E | 0.03* | 40/360 | 0.75 |
| HeLa | HRV A1 | 0.005* | 400/3600 | 0.012 |
| Vero | PV | 0.16 | 100/900 | 1.6 |