Table 2.

The differentially expressed protein lists between PEDV-infected and mock-infected groups.

No.	Protein name	UniProtKB accession no.	GO annotation			P value	Average V/Cb
			Molecular function	Cellular component	Biological process
1	Cystatin-C or Cystatin-3	G7PH52	enzyme regulator activity	–	metabolic process; regulation of biological process	6.98E−04	0.46
2a	Osteopontin precursor	F7F5L5	–	–	–	9.11E−03	0.50
3	Retinol dehydrogenase 10	G7MZK0	nucleotide binding; catalytic activity	cytoplasm; membrane	development; metabolic process; reproduction; cell differentiation	5.22E−03	0.59
4	Testis cDNA clone	Q4R3Z6	–	–	–	1.36E−03	0.59
5	Overexpressed in colon carcinoma 1 protein	H9FAZ7	–	–	–	6.48E−04	0.61
6	Cytochrome b-245 light chain	H9F3U1	–	membrane	–	1.11E−02	0.62
7a	Centrin-2	F7HKU5	metal ion binding; nucleotide binding; catalytic activity	–	–	1.26E−03	0.66
8a	Kidney-specific cadherin	F6VCT3	metal ion binding	membrane	–	6.70E−05	0.67
9a	Putative WD repeat-containing protein 33	F6SBK9	protein binding	nucleus	–	1.36E−03	0.67
10	Apolipoprotein E4	D5G333	–	extracellular	transport; metabolic process	9.85E−03	0.68
11	Receptor-type tyrosine-protein phosphatase T isoform 1	H9F9X9	catalytic activity	membrane	metabolic process	3.56E−03	0.68
12a	Putative neutral and basic amino acid transport protein rBAT-like isoform 3	F7HIT7	catalytic activity	–	metabolic process	8.18E−05	0.69
13a	Mitochondrial ornithine aminotransferase	F7BGF3	catalytic activity	cytoplasm; mitochondrion	metabolic process; cell organization and biogenesis	3.51E−02	0.69
14	Lysosomal protective protein	G7N4N3	catalytic activity	nucleus	metabolic process	2.85E-05	0.70
15a	Putative low-density lipoprotein receptor-related protein 2	F7H113	metal ion binding; protein binding	cytoplasm; endosome; endoplasmic reticulum; Golgi; membrane	metabolic process; transport; cell proliferation; development	1.02E−04	0.70
16	Alpha-adducin isoform c	H9FPQ1	metal ion binding	cytoskeleton	–	3.58E−03	0.71
17	Integrin beta 2	H9Z8N5	receptor activity; protein binding	membrane	cell communication; regulation of biological process; response to stimulus; development	1.82E−04	0.71
18	Dipeptidyl peptidase 2 preproprotein	H9EXB4	catalytic activity	membrane	metabolic process	4.22E−03	0.71
19a	Estrogen sulfotransferase	F6RUQ2	catalytic activity	nucleus; cytoplasm; membrane	metabolic process	4.73E−03	0.71
20a	Putative protein EGK_14077	F6PJM4	–	–	–	2.34E−03	0.72
21a	Putative legumain	F6S082	catalytic activity	cytoplasm; endosome	response to stimulus; metabolic process; regulation of biological process; cell death	2.53E−03	0.73
22	Metallothionein-1E	F6PYY1	metal ion binding	nucleus; cytoplasm	regulation of biological process; response to stimulus	4.86E−03	0.74
23	Trophoblast glycoprotein	H9F4Q1	protein binding	membrane	–	6.68E−04	0.74
24a	Putative proactivator polypeptide isoform X6	F7F376	–	cytoplasm; vacuole	metabolic process	6.40E−03	0.74
25	Cathepsin D (Predicted)	A9L947	catalytic activity	–	metabolic process	1.33E−04	0.75
26	Aldehyde dehydrogenase family 1 member L1	F7HB04	catalytic activity	cytoplasm; mitochondrion	metabolic process	9.07E−03	0.75
27	Carbonic anhydrase 2	G7MZP3	catalytic activity; metal ion binding	–	metabolic process	1.83E−03	0.75
28	Erythrocyte band 7 integral membrane protein isoform a	F7HP19	–	cytoskeleton; membrane; extracellular	cell organization and biogenesis	4.40E−05	0.75
29a	Putative laminin subunit beta-1	F7HPY4	catalytic activity; motor activity; signal transducer activity; protein binding; structural molecule activity; nucleotide binding	extracellular; nucleus; cytoplasm; membrane; cytoskeleton; mitochondrion	metabolic process; regulation of biological process; cell communication; response to stimulus; transport; cellular component movement; development; cell differentiation; cell organization and biogenesis	1.92E−03	0.76
30a	Putative tissue alpha-L-fucosidase	F7HDC0	catalytic activity	–	metabolic process	6.56E−03	0.76
31	Similar to human bone marrow stromal cell antigen 1	I7GP78	catalytic activity	–	–	2.77E−02	0.77
32	Solute carrier family 17, member 5	A9X190	–	membrane	transport	8.00E−03	0.77
33	Galectin	G7N3S9	–	–	–	2.88E−03	0.78
34	Sulfhydryl oxidase 2	H9F332	catalytic activity	membrane	metabolic process	4.97E−03	0.78
35	Folate receptor alpha	F7BP60	–	–	–	2.98E−03	0.78
36a	Putative protein EGK_10171	G7NLB0	–	–	–	4.60E−03	0.79
37	Lysosome-associated membrane glycoprotein 2 isoform B	F7BCK9	–	membrane	–	1.61E−03	0.79
38	RNA-binding motif protein 12B	G7MZR8	nucleotide binding	membrane	–	2.10E−03	0.79
39	Similar to human synaptobrevin-like 1 (SYBL1)	I7G8H0	–	membrane	transport	8.83E−03	0.79
40a	Putative polyadenylate-binding protein-interacting protein 1 isoform 2	F7H0R0	DNA binding; RNA binding; protein binding	–	metabolic process	1.17E−04	0.79
41a	Putative versican core protein-like isoform 8	F7C5T5	metal ion binding; protein binding	extracellular	cell differentiation; cellular component movement; development	5.67E−05	0.79
42a	Putative N-acetylglucosamine-6-sulfatase	G7PIY5	catalytic activity	cytoplasm; vacuole	metabolic process	1.14E−04	0.80
43	ACADSB	Q5IFK6	catalytic activity; nucleotide binding	–	metabolic process	3.47E−02	0.80
44	Clusterin	Q5ISQ2		–	cell death	2.54E−03	0.80
45	Cathepsin Z	G7N4B3	catalytic activity	–	metabolic process	6.96E−03	0.80
46a	Endoplasmic reticulum resident protein 28	F7GNV5	–	cytoplasm; endoplasmic reticulum; organelle lumen	transport	4.49E−02	0.80
47a	Putative transducin-like enhancer protein 4 isoform 12	F6PZC8	protein binding	nucleus	metabolic process; regulation of biological process	2.22E−03	0.81
48a	T-cell immunoglobulin and mucin domain-containing protein 1	F7GB98	protein binding	–	–	4.71E−05	0.81
49a	Putative N-acetylgalactosamine-6-sulfatase	G7NQ90	catalytic activity	–	metabolic process	1.58E−02	0.81
50a	Uncharacterized protein	F7GQ07	catalytic activity	–	metabolic process	3.01E−04	0.81
51a	Putative galactokinase	F7DF76	nucleotide binding; catalytic activity	cytoplasm	metabolic process	4.72E−02	0.81
52	Signal transducer and activator of transcription	Q9N145	protein binding; DNA binding; signal transducer activity	nucleus; cytoplasm; membrane	cell differentiation; development; metabolic process; regulation of biological process; transport; cell communication; response to stimulus; cell proliferation; reproduction	7.92E−03	0.81
53	Delta(14)-sterol reductase	G7PPJ4	catalytic activity	membrane	metabolic process	3.51E−02	0.81
54a	Putative disabled homolog 2 isoform X4	F7GRX9	protein binding	–	–	4.59E−03	0.81
55	Pyridoxal-dependent decarboxylase domain-containing protein 1	F7GW28	catalytic activity	–	metabolic process	1.59E−02	0.81
56	Rho GTPase-activating protein 29	H9FI14	metal ion binding	membrane	cell communication; regulation of biological process; response to stimulus	1.51E−02	0.82
57	Protein DB83	F7H2H1	–	membrane; cytoplasm	–	2.25E−03	0.82
58	DORA reverse strand protein	G7Q0P8	catalytic activity	–	metabolic process	1.01E−02	0.82
59	Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (Cyclizing)	G7PPY4	catalytic activity; nucleotide binding	membrane	metabolic process	4.96E−03	0.82
60	Cadherin 6	Q5ISM2	metal ion binding	membrane	–	6.61E−03	0.82
61a	Putative mitochondrial delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase	F6TNT1	catalytic activity; protein binding	cytoplasm; mitochondrion	metabolic process	5.83E−03	0.82
62	Fatty acid-binding protein, heart	G7MI71	transporter activity	–	transport	2.36E−03	0.82
63a	Putative NADH dehydrogenase 1 α subcomplex subunit 8	F7B3T9	–	cytoplasm; mitochondrion; membrane	–	1.78E−03	0.83
64	Endoplasmic reticulum resident protein 58	G7NJK7	protein binding	cytoplasm; endoplasmic reticulum; organelle lumen	–	5.49E−03	0.83
65	Agrin	H9FU64	transporter activity; protein binding	extracellular; membrane	transport; cell communication; regulation of biological process; response to stimulus; cell organization and biogenesis	9.20E−03	0.83
66a	Toll-interacting protein	F7DRQ6	protein binding	–	–	2.53E−02	0.83
67a	Putative tetraspanin-3 isoform X2	F6SRI3	–	membrane	–	3.37E−03	0.83
68a	Putative protein EGK_14027	G7MM35	enzyme regulator activity; protein binding	cytoplasm; cytoskeleton; membrane	metabolic process; regulation of biological process	6.05E−03	0.83
69	Inosine-5′-monophosphate dehydrogenase	F6VXT4	nucleotide binding; catalytic activity; protein binding; metal ion binding	nucleus; cytoplasm; membrane	metabolic process; cell proliferation; response to stimulus	3.12E−04	1.20
70	78 kDa glucose-regulated protein	F7C3R1	nucleotide binding; catalytic activity; protein binding	nucleus; cytoplasm; endoplasmic reticulum; cell surface; membrane	cell organization and biogenesis; cell communication; regulation of biological process; response to stimulus; metabolic process; development; cell death	9.49E−05	1.20
71a	Uncharacterized protein	F6W6U2	–	–	–	1.40E−02	1.20
72a	Follistatin-related protein 1	G7MKF2	metal ion binding; protein binding	–	–	3.29E−04	1.20
73a	Putative retrotransposon-like protein 1	F7G2J3	RNA binding; catalytic activity	–	metabolic process	6.78E−04	1.20
74a	Heat shock protein 105 kDa	F6S529	nucleotide binding	–	–	6.60E−05	1.21
75a	Putative protein midA homolog	F7GTQ7	–	–	–	2.96E−03	1.21
76	Similar to human S-adenosylhomocysteine hydrolase-like 1	I7GBN2	catalytic activity	–	metabolic process; transport	9.34E−03	1.21
77	Transferrin receptor 1	F6UX47	catalytic activity	extracellular; cytoplasm; endosome; membrane; cell surface	metabolic process; cellular homeostasis; cell differentiation; development; regulation of biological process	4.64E−04	1.22
78a	Exocyst complex component 6B	F7GZR4	–	cytoplasm	transport	5.75E−03	1.22
79	Protein S100-A2	H9F670	metal ion binding	membrane	–	4.75E−02	1.22
80a	Ephrin type-A receptor 2	F7E018	nucleotide binding; catalytic activity; receptor activity; signal transducer activity; protein binding	membrane	development; cell differentiation; metabolic process; cell communication; regulation of biological process; response to stimulus; cell death; cell organization and biogenesis; cell proliferation; cellular component movement	3.02E−02	1.22
81a	Putative nucleolar RNA helicase 2-like isoform 3	F6SQP8	nucleotide binding; RNA binding; catalytic activity	nucleus	–	1.12E−03	1.23
82a	Putative polyadenylate-binding protein 1-like isoform X2	F7EU27	nucleotide binding; RNA binding	–	–	1.77E−03	1.23
83a	Uncharacterized protein	F7CEU8	nucleotide binding	–	–	5.52E−03	1.23
84	Sequestosome-1 isoform 1	I2CY26	metal ion binding	–	–	8.51E−03	1.23
85	NEDD8 ultimate buster 1 isoform 2	H9EZG1	protein binding	–	metabolic process; regulation of biological process; defense response; response to stimulus	2.32E−02	1.23
86	Thioredoxin domain-containing protein 9	F6YEB0	–	nucleus; cytoplasm; cytoskeleton	cellular homeostasis; regulation of biological process	3.79E−02	1.23
87	Four and a half LIM domains protein 2	F7GXH4	protein binding; metal ion binding	nucleus; cytoskeleton	metabolic process; regulation of biological process; cell differentiation; response to stimulus; cell death; development	2.24E−05	1.23
88	Similar to human DKFZP564M182 protein	I7G2J1	RNA binding; structural molecule activity	cytoplasm; ribosome	metabolic process	2.95E−03	1.23
89	Similar to human hypothetical protein FLJ10634	I7GMW8	nucleotide binding	–	–	1.69E−02	1.23
90a	Putative hexokinase-2	F6Y855	nucleotide binding; catalytic activity	cytoplasm; membrane; mitochondrion	metabolic process	3.26E−04	1.24
91	Ribosomal protein L37	F7FYI2	RNA binding; structural molecule activity; metal ion binding	cytoplasm; ribosome; cytosol	metabolic process	3.51E−03	1.24
92	Pyruvate kinase	F7FI39	metal ion binding; catalytic activity	–	metabolic process	1.07E−02	1.24
93	Protein phosphatase 1B isoform 2	H9EM08	metal ion binding; catalytic activity	–	metabolic process	1.40E−02	1.24
94a	Glycogen synthase kinase-3 alpha	G7PXQ8	nucleotide binding; catalytic activity	membrane	metabolic process	4.34E−03	1.24
95	Phosphoserine aminotransferase	F7H2J8	catalytic activity	–	metabolic process	1.01E−03	1.25
96	Histone H2A	H9FCA2	DNA binding; protein binding	chromosome; nucleus; membrane	cell organization and biogenesis; metabolic process	2.02E−03	1.25
97	EF-hand domain-containing protein D2	H9FC53	metal ion binding	–	–	6.88E−04	1.25
98a	Putative ATP-dependent RNA helicase DDX10	F7BIN5	nucleotide binding; catalytic activity	–	–	1.20E−04	1.25
99a	Putative pterin-4-alpha-carbinolamine dehydratase	F7F694	catalytic activity; protein binding	nucleus; cytoplasm	metabolic process; cell organization and biogenesis	1.85E−02	1.26
100	Integrin beta 3	F7FG54	receptor activity; protein binding	membrane; cell surface	cell organization and biogenesis; cell communication; regulation of biological process; response to stimulus; development; cellular component movement; cell proliferation; coagulation	2.60E−04	1.26
101	Similar to human exocyst complex component 7	I7GLB8	–	cytoplasm	transport	5.30E−03	1.27
102a	Putative leucine-rich repeat flightless-interacting protein 1	F6S3D2	–	–	–	1.48E−03	1.27
103a	Uncharacterized protein	G7P460	–	–	–	8.80E−04	1.28
104	Eukaryotic initiation factor 4A-I isoform 1	H9FAB5	nucleotide binding; RNA binding; catalytic activity	cytoplasm	metabolic process	2.51E−03	1.29
105a	Phosphatidylinositol transfer protein beta isoform isoform 2	F7G7C8	–	–	transport	1.70E−02	1.29
106	Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 2	G7NE91	protein binding	–	metabolic process	4.14E−03	1.30
107a	Putative tripartite motif-containing protein 47 (TRIM47)	F7HF49	protein binding; metal ion binding	intracellular	–	2.65E−02	1.31
108	UDP-N-acetylhexosamine pyrophosphorylase	F7CXU2	catalytic activity	–	metabolic process	3.83E−02	1.32
109a	Putative protein EGK_20713	F7B9G5	protein binding	–	–	3.42E−03	1.32
110a	Putative 60S ribosomal protein L23a-like	F7HD49	nucleotide binding; structural molecule activity	cytoplasm; ribosome	metabolic process	3.89E−04	1.33
111	Mitochondrial import inner membrane translocase subunit Tim13	F6R7Z6	metal ion binding	cytoplasm; mitochondrion	cell organization and biogenesis; transport	1.98E−04	1.33
112	Poly [ADP-ribose] polymerase 9 isoform c	H9ZE68	catalytic activity	membrane	–	3.46E−03	1.34
113a	Serpin B6	F7BRQ5	enzyme regulator activity	extracellular	–	7.69E−04	1.35
114	EH domain-containing protein 4	F7HAE5	catalytic activity; metal ion binding; protein binding; nucleotide binding	cytoplasm; endoplasmic reticulum; endosome; membrane	metabolic process; transport; cell organization and biogenesis	2.73E−04	1.35
115	Calponin-like integrin-linked kinase-binding protein	G7PQP7	protein binding; catalytic activity	membrane	cell communication; regulation of biological process; response to stimulus; metabolic process	5.57E−04	1.35
116a	Putative protein EGK_00649	G8F2R2	transporter activity	membrane	transport	2.83E−03	1.36
117	Ribosomal RNA-processing protein 8	F7C3H4	catalytic activity; protein binding	nucleus; organelle lumen; cytoplasm; membrane; chromosome	metabolic process; regulation of biological process; cell communication; response to stimulus; cell death	3.59E−03	1.36
118	Microtubule-associated protein 1B	G7P7P5	protein binding; catalytic activity	cytoskeleton; membrane	cell organization and biogenesis	3.37E−05	1.37
119	FMRP-interacting protein 2	G7NGR9	–	–	–	3.95E−04	1.42
120a	Putative centrosomal protein of 89 kDa isoform X1	F7H5V4	protein binding	–	–	8.26E−04	1.44
121a	Ubiquitin-conjugating enzyme	F6ULI1	catalytic activity	–	–	3.58E−03	1.49
122	Nucleolar complex protein 14	G7NVR8	–	membrane	–	4.14E−07	1.52
123	Ubiquitin-like protein ISG15	F7GS84	protein binding	extracellular	metabolic process; regulation of biological process; defense response; response to stimulus	1.59E−05	1.76
124a	Putative caspase-7 isoform X1	G7PDZ9	catalytic activity	cytoplasm	metabolic process; cell death	2.21E−04	1.76
125a	Putative tRNA pseudouridine synthase Pus10	H9FK14	–	–	–	2.56E−04	2.21
126	OCIA domain-containing protein 1 isoform 2	H9FWA7	–	membrane	–	1.20E−02	2.50

^aThe names of the uncharacterized/putative proteins were annotated again and corrected by the blastp search in GenBank database of NCBI based on the primary identified protein sequences from the UniProtKB.

^bThe average V/C ratio >1 represents the up-regulated proteins; the average V/C ratio <1 represents the down-regulated proteins.