Fig. 2.

rpIFN-L1 and rpIFN-L3 inhibited PEDV infection both in Vero E6 and in IPEC-J2 cells by IFA. Vero E6 (A, B) or IPEC-J2 (C) cells were exposed to the indicated concentrations of rpIFN-L1 (A, C) or rpIFN-L3 (B) for 24 h prior to infection with PEDV, and then cells were infected with PEDV (strain CV777) for 2 h, maintained for 36 hpi without the presence of rpIFN-L, washed, and fixed with 4% paraformaldehyde. The virus was detected with PEDV N protein IFA. Nuclei were visualized by staining with DAPI. Scale bars represent 200 μm.