Table 1.
The inserted sequences in shRNA-expressing plasmids.
| Name | Sequencea |
|---|---|
| BglⅡ-PEDV/SADS-M-EcoRⅠ-F: | 5′-gatcGCTGGAATWTCAYAYGGAATttcaagagaATTCCRTRTGAWATTCCAGTTTTTTg-3′ |
| BglⅡ-PEDV/SADS-M-EcoRⅠ-R: | 5′-aattcAAAAAACTGGAATWTCAYAYGGAATtctcttgaaATTCCRTRTGAWATTCCAGC-3′ |
| XhoⅠ-PDCoV-N-KpnⅠ-F: | 5′-tcgagGGCTGACACTTCTATTAAAttcaagagaTTTAATAGAAGTGTCAGCCTTTTTTggtac-3′ |
| XhoⅠ-PDCoV-N-KpnⅠ-R: | 5′-cAAAAAAGGCTGACACTTCTATTAAAtctcttgaaTTTAATAGAAGTGTCAGCCc-3′ |
| BglⅡ-NC-EcoRⅠ-F: | 5′-gatcGCTAGTATGTGCGTGCGGTTttcaagagaAACCGCACGCACATACTAGTTTTTTg-3′ |
| BglⅡ-NC-EcoRI-R: | 5′-aattcAAAAAACTAGTATGTGCGTGCGGTTtctcttgaaAACCGCACGCACATACTAGC-3′ |
| XhoⅠ-NC-KpnⅠ-F: | 5′-tcgagCTAGTATGTGCGTGCGGTTttcaagagaAACCGCACGCACATACTAGTTTTTTggtac-3′ |
| XhoⅠ-NC-KpnⅠ-R: | 5′-cAAAAAACTAGTATGTGCGTGCGGTTtctcttgaaAACCGCACGCACATACTAGc-3′ |
a
Lowercase letters indicated the end sequences of the enzyme digestion reaction; Underlined lowercase letters indicated the loop sequences; Uppercase letters indicated the siRNA sequences. W: A or T; Y: C or T; R: A or G.