Table 2.
Comparative results of clinical samples tested by conventional RT-PCR, SYBR green-based RT-qPCR and probe-based RT-qPCR for SADS-CoV detection.
| Samples | Number of samples | Number of SADS-CoV positive samples |
||
|---|---|---|---|---|
| Conventional RT-PCR | SYBR-based RT-qPCR | probe-based RT-qPCR | ||
| Heart | 10 | 3 (13.36–26.16)a | 5 (13.36–33.67)b | 5 (18.28–34.41)c |
| Lung | 10 | 4 (14.61–25.23) | 6 (14.61–34.45) | 6 (20.23–34.98) |
| Liver | 10 | 4 (15.06–28.63) | 7 (15.06–33.23) | 7 (20.83–34.04) |
| Spleen | 10 | 3 (17.23–26.18) | 7 (17.23–32.54) | 7 (21.35–33.78) |
| Kidney | 10 | 4 (11.57–27.63) | 6 (11.57–31.67) | 6 (16.73–32.39) |
| Jejunum | 10 | 8 (9.36–28.58) | 8 (9.36–28.58) | 8 (15.28–30.35) |
| Duodenum | 12 | 9 (8.67–27.78) | 11 (8.67–29.67) | 11 (14.16–31.41) |
| Ileum | 12 | 10 (9.17–28.58) | 12 (9.17–30.24) | 12 (14.79–31.89) |
| Total | 84 | 45 | 62 | 62 |
a
Ct values of the positive samples in both SYBR green-based RT-qPCR and conventional RT-PCR.
b
Ct values of the positive samples in SYBR green-based RT-qPCR.
c
Ct values of the positive samples in probe-based RT-qPCR.