Fig. 4.

CLR01 inactivates epidemic ZIKV isolates. (A) ZIKV FB-GWUH-2016 or (B) PRVABC-59 was incubated for 10 min at 37 °C with 0.2-150 μM CLR01 or CLR03. Thereafter, Vero E6 cells were infected and 2 days later, infection rates were determined via a cell-based ZIKV immunodetection assay. Data points represent mean ± SD (n = 3). IC₅₀ values were determined with GraphPad Prism. One-way ANOVA (non-parametric, grouped), followed by Bonferroni's multiple comparison tests were applied to compare the different CLR01 concentrations to cells infected in the absence of compound (** denotes p < 0.001; *** denotes p < 0.0001).