Table 2.

Limit of detection for CDC and PHL viral respiratory LDTs in 96 well format and commercial multi-respiratory panels. Results that did not meet the acceptance criteria (at least 19 out of 20 replicates tested positive for detection) were considered indeterminate. LoD is expressed as the highest dilution (lowest concentration) of extracted reference standard that met acceptance criteria for the experiment.

Column	A	B	C	D	E	F	G	H	I	J	K	L
State PHL	IA		WA		MA	WI	HI	NY		MA	WI	HI
Assay type	CDC	CDC	CDC	CDC	CDC	CDC	CDC	CDC	LDT	TessArray RPM Flu 3.1	Qiagen Resplex II v2	Luminex xTAG
Instrumentation 7500 = ABI 7500 Fast ViiA7 = ABI ViiA7 IQ5 = Bio-Rad IQ5	7500	ViiA7	7500	ViiA7	ViiA7	ViiA7	ViiA7	ViiA7	IQ5	Affymetrix Genechip	Luminex 200	Luminex 200
Adenovirus	−5	a	−4	−4	−5	NA	−5	−5	−5	−3	NA	−4
Human coronavirus 229E	NA	NA	−5	−5	−5	−5	NA	−6	−6	−2	−5	NA
Human coronavirus NL63	NA	NA	−6	−6	−6	−6	NA	−7	−6	−3	−4	NA
Human coronavirus OC43	NA	NA	−5	−5	−5	−4	NA	−6	−6	−2	−4	NA
Human metapneumovirus A1	−5	−5	−5	−5	−4	−5	−5	−5	−5	d	−3	−4
Human parainfluenza virus 1	−4	−4	NA	NA	−3	−3	NA	−3	−1	−1	−3	NA
Human parainfluenza virus 2	−6	−6	NA	NA	−5	c	NA	−7	−7	−3	−5	NA
Human parainfluenza virus 3	−6	−6	NA	NA	−6	−6	NA	−6	−7	−2	−4	NA
Human parainfluenza virus 4	NA	NA	−3	−3	−2	−3	NA	−3	−3	−1	−1	NA
Respiratory enterovirus (CoxA9)	NA	NA	−6	−6	−6	−6	NA	NA	NA	−5	−7	NA
Respiratory syncytial virus	−6	−6	NA	NA	−5	−6	−6	−6	−7	−3	−3	−3
Rhinovirus	−5	b	−5	−5	−6	−6	−5	−6	−6	ND	−4	−5

a – 12/20 positive (60%) detection at −6.

b – 17/20 positive (85%) detection at −5.

c – 12/20 positive (60%) detection at −6.

d – 1/10 positive (10%) detection at −1.

NA – assay not assigned to site.

ND – panel not designed to detect Rhinovirus.