Table 4.
Number of positive results of the QPrimer-based rRT-LAMP assay in the detection of each in vitro-transcribed standard RNA.a
| Target | Standard RNA | Concentration of RNA (copies/reaction) |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| 5000 | 500 | 250 | 50 | 25 | 5 | 0.5 | N.C. | ||
| IAV | A/Narita/1/2009 (H1N1)pdm09 M gene | 3 | 3 | 3 | 1 | 1 | 0 | 0 | 0 |
| IBV | B/Massachusetts/2/2012 NS gene | 3 | 3 | 3 | 2 | 1 | 0 | 0 | 0 |
| A/H1pdm | A/Narita/1/2009 (H1N1)pdm09 HA gene | 3 | 3 | 3 | 2 | 1 | 0 | 0 | 0 |
| A/H3 | A/Texas/50/2012 (H3N2) HA gene | 3 | 2 | 2 | 0 | 0 | 0 | 0 | 0 |
| RSV A | RSV/OsakaC.JPN/16.2012 N gene | 3 | 3 | 2 | 2 | 0 | 0 | 0 | 0 |
| RSV B | RSV/OsakaC.JPN/38.2011 N gene | 3 | 2 | 2 | 0 | 0 | 0 | 0 | 0 |
a
The assays were carried out in triplicate at each concentration.