Table 2.
Percentage of positive samples by different diagnostics assays.
| Animal species | Assays |
||||
|---|---|---|---|---|---|
| EM | ELISAa | Commercial RT-iiPCR | Commercial real-time rtRT-PCR kitb | In-house rtRT-PCR assay | |
| Bovine | 21/68 (30.9%) | 20/54 (37%) | 54/76 (71%) | 52/76 (68.4%) | 35/76 (46%) |
| Equine | 1/6 (16.7%) | 2/21 (9.5%) | 3/22 (13.6%) | 2/22 (9.1%) | 2/22 (9.1%) |
| Caprine | 1/4 (25%) | 0/4 (0%) | 1/4 (25%) | 0/4 (0%) | 0/4 (0%) |
| Canine | 3/6 (50%) | 3/6 (50%) | 4/6 (66.7%) | 3/6 (50%) | 3/6 (50%) |
| Total | 26/84 (31%) | 25/85 (29.4%) | 62/108 (57.4%) | 57/108 (52.8%) | 40/108 (37%) |
a
Premier™ Rotaclone ELISA kit, Meridian Bioscience, USA.
b
LSI VetMAX™ triple ruminant Rotavirus and Coronavirus Real-Time PCR kit, Thermo Fisher Scientific, USA.