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Percentage agreement between positive samples by Commercial Real-time rtRT-PCR kita versus other assays, broken down by rtRT-PCR Ct values.
| Ct Range | Assays |
|||
|---|---|---|---|---|
| EM | ELISAb | Commercial RT-iiPCR | In-house rtRT-PCR assay | |
| 5–15 | 100% | 100% | 100% | 100% |
| 16–25 | 81.3% | 100% | 100% | 94% |
| 26–35 | 12% | 40% | 100% | 83.3% |
| 36–45 | 0% | 22.2% | 87% | 9% |
LSI VetMAX™ triple ruminant Rotavirus and Coronavirus Real-Time PCR kit, Thermo Fisher Scientific, USA.
Premier™ Rotaclone ELISA kit, Meridian Bioscience, USA.
