Fig. 3.
Proteolytic inhibition of PEDV PL2proby 6TG. Peptidyl substrate at concentrations of 10–50 μM and 6TG at concentrations of 0–60 μM were used for the measurements, while the protein concentration was held at 3.85 μM. The circles, squares and triangles represent mean values, while the bars represent the standard error. The solid lines show the best-fit results in accordance with a noncompetitive inhibition model (Eq. (2)) and Rsqr value is 0.964. The kinetic parameters from the best fit are shown in Table 1. The assay was repeated to ensure reproducibility.