. 2018 Aug 20;158:199–205. doi: 10.1016/j.antiviral.2018.08.011

Table 1.

Steady-state apparent kinetic parameters of PEDV PL2^pro and its T39W mutant.

Peptidyl substrate	PEDV PL2^pro	K_m (μM)	k_cat (min⁻¹)	k_cat/K_m (μM ⁻¹min⁻¹)
Dabcyl-FRLKGGAPIKGV-Edans	Wild-type	18.6 ± 4.3	0.065 ± 0.006	0.0035
Dabcyl-FRLKGGAPIKGV-Edans	T39W	32.1 ± 12	1.050 ± 0.198	0.0327
Dabcyl-FKKKGGGDVKE-Edans	Wild-type	61.6 ± 19.7	0.299 ± 0.061	0.0049
Dabcyl-FKKKGGGDVKE-Edans	T39W	39.6 ± 15.6	0.247 ± 0.053	0.0062

The steady-state apparent kinetic data for hydrolyzing either Dabcyl-FRLKGGAPIKGV-Edans (SARS-CoV-derived) or Dabcyl-FKKKGGGDVKE-Edans (PEDV-derived) substrates were determined according to the Michaelis-Menten equation. R_sqr were 0.964, 0.938, 0.976, and 0.944.