Table 2.
Inhibition of PEDV PL2pro by various compounds.
| Name | Structure | IC50 (μM)a | Kis (μM)b |
|---|---|---|---|
| 6TG |  |
13.7 ± 1.7 | 21.1 ± 1.1 |
| 6MP |  |
58.1 ± 5.7 | – |
| Hypoxanthine |  |
NDc | – |
| 2-amino-6-methyl-mercaptopurine |  |
NDc | – |
| Mycophenolic acid |  |
>200 | – |
| Disulfiram |  |
>100 | – |
| GRL0617 |  |
>100 | – |
a
The DUB activity assay was used for the measurement of IC50 (Eq. (1)).
b
Proteolytic activity at various concentrations of SARS-CoV-derived peptidyl substrate and 6TG was measured (Fig. S6 and Fig. 3) and the apparent Kis was determined from the best global fit of the data to a noncompetitive inhibition model (Eq. (2)). Rsqr was 0.964.
c
ND: IC50 was not determined due to lack of inhibition at a compound concentration of 200 μM.