Table 2.
Numbers of positive samples in 117 clinical specismens from sick pigs detected for earch of the four viruses by multiplex RT-PCR and TaqMan qRT-PCRa.
| Virus detected | Positive samples in multiplex RT-PCR | Positive samples in single RT-PCR | Positive samples in Taqman qRT-PCR | 
|---|---|---|---|
| CSFV only | 10 | 10 | 11 | 
| PRRSV only | 23 | 23 | 25 | 
| PEDV only | 16 | 16 | 17 | 
| TGEV only | 2 | 2 | 2 | 
| CSFV/PRRSV | 5 | 5 | 7 | 
| PEDV/PRRSV | 1 | 1 | 1 | 
| No virus | 60 | 60 | 54 | 
Taqman qRT-PCR was developed by our laboratory based on previous reports (Chen et al., 2004; Lurchachaiwong et al., 2008; Risatti et al., 2003; Wang et al., 2014).