Fig. 3.

Reduction of the influenza viral protein level in cells by the active dihydrofuropyridinones. MDCK cells were mock-infected (lane 1) or infected with PR8 virus (lanes 2–15), and then incubated with either DMSO (lane 2), different concentrations (11, 33, and 100 μM) of 15a (lanes 3–5), 15g (lanes 6–8), 15j (lanes 9–11), or 15q (lanes 12–14), or 11 μM OSV-C (lane 15) for 24 h at 35 °C. The viral proteins, NP and HA, were detected using their specific primary antibodies and HRP-conjugated secondary antibodies. Cellular β-actin was used as a loading control. Each protein is indicated with an arrow at the right of the panel. The triangles at the top indicate an increase in the chemical concentration.