Table 6.
The coefficients of variation of positive and negative sera between runs.a
Number of antisera |
Ib (S/P value) |
IIb (S/P value) |
IIIb (S/P value) |
X | SD | CV (%) |
---|---|---|---|---|---|---|
1 | 1.132 | 0.944 | 0.852 | 0.976 | 0.143 | 14.63 |
2 | 0.910 | 0.958 | 0.790 | 0.886 | 0.086 | 9.78 |
3 | 0.290 | 0.254 | 0.280 | 0.275 | 0.019 | 6.82 |
4 | 0.504 | 0.479 | 0.398 | 0.460 | 0.055 | 11.97 |
5 | 0.522 | 0.451 | 0.394 | 0.455 | 0.064 | 14.09 |
6 | 0.339 | 0.299 | 0.265 | 0.301 | 0.037 | 12.24 |
7 | 0.366 | 0.348 | 0.284 | 0.333 | 0.043 | 13.02 |
8 | 0.491 | 0.449 | 0.425 | 0.455 | 0.033 | 7.33 |
9 | 0.563 | 0.479 | 0.530 | 0.524 | 0.042 | 7.99 |
10 | 0.416 | 0.404 | 0.531 | 0.451 | 0.070 | 15.53 |
11 | 0.072 | 0.099 | 0.097 | 0.089 | 0.015 | 16.71 |
12 | 0.060 | 0.055 | 0.069 | 0.061 | 0.007 | 11.88 |
13 | 0.098 | 0.094 | 0.084 | 0.092 | 0.008 | 8.31 |
14 | 0.088 | 0.075 | 0.099 | 0.088 | 0.012 | 14.07 |
15 | 0.073 | 0.059 | 0.063 | 0.065 | 0.007 | 11.51 |
The negative–positive cut-off was 0.12.
Three repeated experiments with different batches of antigens.