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. 2019 Nov 8;275:113771. doi: 10.1016/j.jviromet.2019.113771

Table 2.

Repeatability of the RT-qPCR assay for differentiation between vaccine Poulvac IB QX and QX-like field strain. Mean Ct (three replicates), standard deviation (SD) and CV are reported for each virus according to virus, dilution, operator and week of experiment.

Week 1
Week 2
Week 3
Total
Virus Titre Operator Ct mean ± SD CV Ct mean ± SD CV Ct mean ± SD CV Ct mean ± SD CV
Poulvac IB QX 106.83 1 17,00 ± 0,14 0,80 16,93 ± 0,13 0,77 16,89 ± 0,15 0,90 16,77 ± 0,30 1,79
2 16,82 ± 0,20 1,21 16,66 ± 0,63 2,18 16,34 ± 0,31 1,89
104.83 1 24,65 ± 0,12 0,85 23,88 ± 0,25 1,03 24,52 ± 0,07 0,29 24,36 ± 0,49 2,01
2 24,81 ± 0,45 1,82 23,67 ± 0,17 0,72 24,66 ± 0,21 0,86
102.83 1 32,33 ± 0,53 1,64 33,61 ± 0,37 1,11 32,12 ± 0,18 0,55 32,05 ± 0,93 2,90
2 31,70 ± 0,53 1,68 31,20 ± 0,87 2,79 31,31 ± 0,03 0,08
IBV-D388 106.31 1 17,75 ± 0,23 1,30 17,72 ± 0,17 0,95 17,57 ± 0,16 0,93 17,70 ± 0,38 2,14
2 17,50 ± 0,28 1,58 17,37 ± 0,39 2,25 18,31 ± 0,26 1,40
104.31 1 25,26 ± 0,42 1,64 24,63 ± 0,45 1,82 25,71 ± 0,47 1,86 24,98 ± 0,43 1,72
2 25,26 ± 0,38 1,51 24,77 ± 0,25 1,01 24,82 ± 0,44 1,79
102.31 1 33,26 ± 0,14 0,42 32,80 ± 0,30 0,91 31,39 ± 0,72 2,30 32,18 ± 0,93 2,90
2 32,36 ± 0,81 2,51 31,35 ± 0,89 2,85 32,23 ± 0,89 2,77