Table 3.

Comparison of X-Tag analytical sensitivity to qRT-PCR.

(3.1) qRT-PCR and 8 Plex assaya
Assay	qPCR Cedar P-gene	X-Tag Cedar P-gene	qPCR Nipah N-gene	X-Tag Nipah N-gene
	Ct	MFI	Ct	MFI
10-1	29	21,994	23	21,367
10-2	33	21,417	27	21,498
10-3	37	17,941	30	19,103
10-4	40	6415	33	8847
10-5	Ub	208	36	953
10-6			Ub	121

(3.2) qRT-PCR and 11 Plex assaya
Assay	qPCR Cedar P-gene	X-Tag Cedar L-gene	qPCR HeV N-gene	X-Tag HeV L-gene
	Ct	MFI	Ct	MFI
10-1	27	19,078	24	19,332
10-2	31	2483	28	18,569
10-3	35	257	31	13,774
10-4	39.5 (1/2)	235	35	4593
10-5	Ub		Ub	1264
10-6				153

^aAbbreviations: U = undetected, MFI = median fluorescence intensity; results are in duplicate. All positive results are shaded in gray (MFI values 676 or above are considered positive).

^bqRT-PCR cut-offs were Ct ≤ 40 positive, ≥45 negative, 40–45 indeterminate.