Fig. 1.

Generation of an EBOV-GP-V pseudotyped virus system. (A) Schematic representation of the 3 co-transfected plasmids, including the lentiviral vectors encoding for the Gaussia luciferase (Gluc) gene, the HIV-1 helper construct Δ8.2 and the pCAGGS-ZEBOV-GP plasmid. (B) Western blot showing the GP1, preGP and P24 protein expression in virions and transfected cells. (C) Equal amounts of GP + or GP- virions (adjusted by P24) were used to infect HEK293T cells. After 48 h, the Gaussia Luciferase activity (Gluc) of the supernatants was measured and normalized as a percentage of the positive control (Gluc value of GP + as 100%) (D) Dose-dependent 2G4 (MAb) inhibition of EBOV-GP-V transduction in HEK293T cells. The same amount of EBOV-GP-V was incubated with the indicated concentration of 2G4 for 30 min and added to HEK293T cells for 4 h. The cells were washed and cultured in complete DMEM without antibody. Gluc activities in the supernatant were tested after 48 h of incubation. (E) Evaluating the transduction ability of EBOV-GP-V in different cell types. The results are the mean values ± standard deviations (SD) of three independent experiments.