Fig. 5.

Analysis of the inhibitory effect of the combination of 2G4 and CHPV on EBOV entry. (A) Various concentrations of 2G4 alone or with CHPV (10 or 0.625 μg/ml) were added to HEK293T cells with the same amount of EBOV-GP-V. The anti-EBOV effect was displayed as percent inhibition, a ratio of Gluc activity in the presence of 2G4 or/and CHPV versus EBOV-GP-V alone. (B) The inhibitory effect of CHPV in the presence or absence of 2G4 in macrophages. The positive control (PC) was infected macrophages in the absence of CHPV or 2G4. EBOV-GP-V was mixed with CHPV (20 ug/ml) in the presence of absence of 2G4 (16 or 1.6 ug/ml) and immediately added onto the cell culture, followed by washing of the cells after 4 h of incubation. The cells were then cultured in medium without CHPV. The supernatants were collected at 24 and 48 h post-infection and used to detect Gluc activity. The major scales of the Y-axis on the left and on the right are different, with 0.5 units and 1 unit, respectively. (C) The same amounts of EBOV-GP-Vs in the presence or absence of 2G4 (1.6 ug/ml) were added onto CHPV (20 μg/mL)-preincubated (1 h) macrophages, and the same amount of CHPV with or without 1.6 ug/mL 2G4 was maintained during the course of infection. The positive control (PC) was infected macrophages in the absence of CHPV or 2G4. The supernatants were collected, and the Gluc activity was tested after 48 h of continuous culture without washing. The major scale base of the Y-axis is 0.5 units on the left and 2 units on the right.