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. 2020 Mar 24;12:2185–2194. doi: 10.2147/CMAR.S234586

Figure 1.

Figure 1

USP8 regulates the growth of cholangiocarcinoma cells. Cholangiocarcinoma cell lines QBC939 and RBE were transfected with siRNA-USP8 to down-regulate its expression, and siRNA negative control was used as negative control group (si-NC); Hucct-1 cells were transfected with pcDNA3.1-USP8 (USP8-OE) to up-regulate its expression, and pcDNA3.1 vector was used as negative control (NC-OE). (A) RT-PCR assay was used to examine the relative expression of USP8 mRNA in QBC939 and RBE cells transfected with siRNAs for 24h. *P<0.05, **P<0.01 by Student’s t-test vs negative control; n = 3. (B) Expression of USP8 mRNA in Hucct-1 cells after transfected withpcDNA3.1-USP8. **P<0.01by Student’s t-test vs negative control; n = 3. (C) The protein expression of USP8 was detected using Western blot analysis. *P<0.05, **P<0.01by Student’s t-test vs negative control; n = 3. (D–E) CCK8 assay was performed to assess QBC939 (D), RBE (E) and Hucct-1 (F) cells proliferation after indicated treatment. *P<0.05 by Student’s t-test vs negative control; n = 3. (G and H) Colony formation assay was used to examine the clonogenic ability of cholangiocarcinoma cells. **P<0.01by Student’s t-test vs negative control; n = 3.