Table 1.
Demographics and clinical outcome of the additional six patients included for MinION Nanopore sequencing analysis of 16S rRNA gene.
| Patient 1 | Patient 2 | Patient 3 | Patient 4 | Patient 5 | Patient 6 | |
|---|---|---|---|---|---|---|
| Demographics | ||||||
| Age (years) | 33 | 65 | 23 | 29 | 53 | 41 |
| Gender | Male | Male | Female | Male | Male | Female |
| Origin | BP | BT | BP | NT | BT | TN |
| Illness day at enrollment (days) | 5 | 3 | 1 | 15 | 4 | 2 |
| Length of hospital stay (days) | 17 | 5 | 12 | 15^ | 13 | 15 |
| Clinical signs/symptoms | ||||||
| Body temperature ( °C) | 37 | 38 | 38 | 37 | 37.2 | 37 |
| Cranial nerve palsy | N | N | N | Y | N | N |
| Hemiplegia/paresis | N | N | N | N | N | N |
| Paraplegia/paresis | N | N | N | N | N | N |
| Tetraplegia/paresis | N | N | N | N | N | N |
| Generalized convulsions | N | N | N | N | N | N |
| Localized convulsions | N | N | N | N | N | N |
| Neck stiffness | Y | Y | Y | Y | Y | N |
| GCS at enrolment | 14 | 14 | 13 | 13 | 9 | 12 |
| CSF examinations | ||||||
| CSF white cell counts | 51,810 | 1609 | 3111 | 1126 | 16,744 | 4760 |
| CSF neutrophils (%) | 78 | 95 | 94 | 60 | 65 | 88 |
| CSF lymphocytes (%) | 22 | 5 | 6 | 40 | 35 | 12 |
| CSF/blood glucose ratio | 0.11 | 0.64 | 0.014 | 0.42 | 0.32 | 0.028 |
| CSF lactate | 11.4 | 9.21 | 12.45 | 5.82 | 13.94 | 15.62 |
| Total protein | 1.33 | 1.133 | 4.731 | 1.37 | 3.861 | 5.746 |
| Routine microbial investigations | ||||||
| ZN smear | ND | ND | Negative | Negative | ND | ND |
| India Ink stain | Negative | ND | Negative | Negative | ND | ND |
| Cryptococcal antigen test | ND | ND | ND | Negative | ND | ND |
| Gram stain | Gram-positive cocci | Gram-positive cocci | Negative | Negative | Negative | Negative |
| Bacterial culture | S. pneumoniae | S. suis | N. meningitidis | Negative | Negative | Negative |
| Bacterial PCR | S. pneumoniae | S. suis | N. meningitidis | S. pneumoniae | S. suis | N. meningitidis |
| MinION 16S rRNA sequencing | ||||||
| 20 min | S. pneumoniae | S. suis | Neisseria | S. pneumoniae | S. suis | Neisseria |
| 1 h | S. pneumoniae | S. suis | Neisseria | S. pneumoniae | S. suis | Neisseria |
| 2 h | S. pneumoniae | S. suis | Neisseria | S. pneumoniae | S. suis | Neisseria |
| GCS at discharge | 15 | 14 | 15 | 14 | 14 | 15 |
Notes toTable 1: GCS: Glasgow Coma Score, BT: Ben Tre, BP: Binh Phuoc, TN: Tay Ninh, NT: Ninh Thuan, HCMC: Ho Chi Minh City, BM: bacterial meingitis, TBM: tuberculous meningitis; N: no, Y: yes; ND: not done.