Table 1.

Oligonucleotides used for RV SIBA assay.

Name	Sequence 5'→3'
RV-A F-primer	TGCACTAGCTGCAGGGTTA
RV-A R-primer	GTGTGCTCACTTTGAG
RV-B F-primer	GTCTCAAGGCTCCAGGGTTT
RV-B R-primer	GTGTGCTTAATTCTGAG
RV-A IO	CCCCCCCCCCCCCCCAGGGTTAAGGTTAGCCACATTCAGGGGmCmCmGmGmAmGmGmAmCmUmCmA
RV-B IO	CCCCCCCCCCCCCCCAGGGTTTAGGTTAGCCGCATTCAGGGGmCmCmGmGmAmGmGmAmCmUmCmA

For invasion oligonucleotide (IO), bold sequences denote non-homologous seeding regions. mA, mC, mG, and mU denote 2′-O-methyl RNA nucleotides. F, forward; R, reverse; RV, human Rhinovirus; SIBA, strand invasion–based amplification; RV-A assay is designed to detect the sequence between position 421 and 483 within the genome of the human rhinovirus 60 strain ATCC VR-1473 (GenBank: FJ445133.1); RV-B assay is designed to detect the sequence between position 429 and 493 within the genome of the human rhinovirus 17 (GenBank: AF542419.1).