Table 2.
Analytical sensitivity and average detection time of RT-SIBA vs. RT-PCR for the detection of RV.
RNA copy number/ reaction | RT-SIBA |
RT-PCR |
||
---|---|---|---|---|
Average amount of time taken to achieve positive results (min) |
Average cycle threshold to achieve positive results (Ct) |
|||
RV-A | RV-B | RV-A | RV-B | |
105 | 20 | 20 | 25 | 22 |
104 | 23 | 24 | 29 | 26 |
103 | 25 | 28 | 34 | 29 |
102 | 28 | 31 | ND | 33 |
101 | 40 | 34 | ND | ND |
0 | ND | ND | ND | ND |