Fig. 4.

Effects of intracellular 2H6-scFv expression on the replication of influenza A virus. (A) Multiple-step growth curve of PR8 (0.01 MOI) in FLAG-tagged 2H6-scFv or 7G12-scFv expressing A549 cells was determined by plaque forming unit at 12, 24, and 48 hpi. (B) 293T cells expressing Myc-tagged 2H6-scFv or 7G12-scFv were infected by PR8 (2 MOI) and harvested at 4, 8, and 12 hpi. Western blot analysis was performed by using anti-M1, anti-Myc and anti-actin antibodies. (C) The expression levels of M1 were showed in graphs after normalization with actin. All experiments were performed in triplicate, and the average values with standard deviations are plotted. Differences in M1 expression between 2H6-scFv and vector transfected cells or between 2H6-scFv and 7G12-scFv transfected cells were evaluated by unpaired t-test (^∗, p < 0.05; ^∗∗∗, p < 0.001).