Table 2.
Comparison of the analytical specificity of two real time RT-qPCRs targeting UTR (Callison et al., 2006) or N (this study) using a panel of IBV and other coronaviruses as well as other avian viral pathogens.
| Virus Strain | RT-qPCR N | RT-qPCR ORF1a |
|---|---|---|
| IBV-M41 | +a | + |
| IBV-Ma5 | + | + |
| IBV-H120 | + | + |
| IBV-H52 | + | + |
| IBV-QX | + | + |
| IBV-QX-like | + | + |
| IBV-CR88-121 | + | + |
| IBV-4/91 | + | + |
| IBV-D880 | + | + |
| IBV-Beaudette | + | + |
| IBV-D274 | + | + |
| IBV-Egyptian Var I | + | + |
| IBV-Egyptian variant II | + | + |
| Turkey coronavirus | + | + |
| Porcine Epidemic Diarrhea | −b | – |
| Transmissible Gastroenteritis | – | – |
| Equine Torovirus | – | – |
| Canine Coronaviruses | – | – |
| Bovine Coronaviruses | – | – |
| H5N1 | – | – |
| H9N2 | – | – |
| NDV | – | – |
a
positive, Cq values obtained with the two RT-qPCRs did not differ by more than 1–2 values indicating similar analytical sensitivity.
b
negative; Cq values > 40.